Engineering Herbicide-Tolerance Rice Expressing an Acetohydroxyacid Synthase with a Single Amino Acid Deletion
Shanghai Academy of Agricultural Sciences
Partner institutions
Shanghai Academy of Agricultural Sciences
Shanghai, China
A comprehensive agricultural research institution in Shanghai, China, spanning crop breeding, food science, and agricultural technology.
How Vibe analyzers were used
A Vibe QM3 rice analyzer measured 1000-grain weight, grain width, and grain length on engineered herbicide-tolerant rice lines to confirm the AHAS mutation did not compromise grain quality.
Study overview
Acetohydroxyacid synthase (AHAS) is an essential enzyme in branched-chain amino acid biosynthesis and a target for herbicide tolerance engineering in crops. This study engineered a herbicide-tolerant rice line expressing an AHAS variant with a single amino acid deletion (AHAS-ΔW548), characterizing its enzymatic and structural properties alongside field performance. Panicles from individual plants were collected to determine seed-setting rate, grains per panicle, and yield per plant, and a Vibe QM3 rice analyzer system was used to measure 1000-grain weight, grain width, and grain length, confirming the engineered herbicide tolerance did not come at the cost of grain size or yield-related grain traits.
Topics and keywords
Related crop analysis
Bibliographic details
Year: 2020
Publication: International Journal of Molecular Sciences
DOI: 10.3390/ijms21041265
Institutions: Shanghai Academy of Agricultural Sciences
More research using Vibe
Explore additional studies applying Vibe analyzers in grain and seed phenotyping workflows.
